Mercodia kits are based strictly on stable, monoclonal antibodies combined with other top quality reagents. This ensures safe long-term production with minimal lot-to-lot variation. By the careful selection of reagents, Mercodia products provide accurate results by first run.
The links underneath will lead you through the principle of the ELISA technology, the advantages of monoclonal antibodies and the accuracy of Mercodia´s assays. You will learn more about the hook effect and sensitivity and how to calculate your results. And last but not least how you can establish your own controls.
ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) is a widely used biochemical technique for the detection of an antigen in a sample.
Sensitivity is a statistical term used to describe the ability of an assay to separate the blank from the specific analyte. Since there are a variety of methods used for determining sensitivity, the method must be known when comparing sensitivity between assays.
The routine analysis of controls with known concentrations is a valuable tool for the acceptance of test runs and to validate that analyzed sample concentrations are in fact repeatable and reproducible at your laboratory.
A robust and well-characterized insulin assay is essential in a variety of different areas. Mercodia's insulin assays for human, rodent and large animal samples have been used for more than 25 years and are great research tools as evident by the vast number of peer-reviewed articles that have been published.
Inaccurate glucagon readings can have enormous negative implications on research and clinical decisions, in this section we will tell you more about what distinguishes the Mercodia Glucagon ELISAs from all other glucagon ELISAs, and give you some tips on what to look for in a good glucagon assay.
It is well known that glucagon acts on the liver to stimulate glycogenolysis and gluconeogenesis. In addition to this, glucagon also regulates amino acid metabolism and ureagenesis and has an effect on hepatic fat metabolism.
Given the suggested patho-physiological significance of oxLDL, there has been a significant increase in the research of the pathogenic role of circulating oxLDL, giving new insights and questions in regards to the role of oxLDL in cardiovascular disease.