Canine Insulin ELISA

(10-1203-01)

Kit format: 1 x 96 wells

Measurement range: 2.3 - 173 mU/L

Specially optimized for canine samples

Highly specific, no cross-reactivity to proinsulin, C-peptide or insulin analogs

Controls available: 10-1221-01

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Description

Mercodia Canine Insulin ELISA

A species-optimized, high quality immunoassay for the quantification of canine insulin in serum or plasma.

Mercodia Animal Insulin Control (10-1221-01) is designed to be used as a three-level control for Canine Insulin ELISA.

Test characteristics

Species

Canine

Sample types

Serum and plasma

Sample volume

25 µL

Assay range

2.3 - 173 mU/L

Detection limit

≤1.15 mU/L

Incubation

2 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Porcine Insulin

100 %

Porcine C-peptide

< 0.001 %

Porcine proinsulin

< 0.2 %

Equine insulin

100 %

Human insulin

108 %

Human C-peptide

< 0.01 %

Human proinsulin

< 0.1 %

NovoRapid

3.2 %

Levemir

Not detected

Lantus

27 %

Humalog

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36112	DfU - 10-1203-01 v. 10.0
35701	DfU - 10-1203-01 v. 10.0
33757	DfU - 10-1203-01 v. 9.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36112	CoA - 10-1203-01
35701	CoA - 10-1203-01
33757	CoA - 10-1203-01

For other languages and previous versions: Technical Library

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references

Brevini, T. A. L., Pennarossa, G., Acocella, F., Brizzola, S., Zenobi, A., & Gandolfi, F. (2016). Epigenetic conversion of adult dog skin fibroblasts into insulin-secreting cells. Veterinary Journal, 211, 52–56. Read more >

Czernichow, P., Reynaud, K., Kerr-Conte, J., Furthner, E., & Ravassard, P. (2019). Production, Characterization, and Function of Pseudoislets from Perinatal Canine Pancreas. Cell Transplantation, 096368971986900. Read more >

Thompson, E. M., Sollinger, J. L., Opara, E. C., & Adin, C. A. (2018). Selective Osmotic Shock for Islet Isolation in the Cadaveric Canine Pancreas. Cell Transplantation, 27(3), 542–550. Read more >