Equine Insulin ELISA

(10-1205-01)

Kit format: 1 x 96 wells

Measurement range: 2.3 - 173 mU/L

Optimized for equine samples

Useful tool for laminitis investigations

Controls available: 10-1221-01

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Description

Mercodia Equine Insulin ELISA

A species-optimized, high quality immunoassay for the quantification of equine insulin in serum or plasma.

Mercodia Animal Insulin Control (10-1221-01) is designed to be used as a three-level control for Equine Insulin ELISA.

Test characteristics

Species

Equine

Sample types

Serum, EDTA, heparin and citrate plasma

Sample volume

25 µL

Assay range

2.3 - 173 mU/L

Detection limit

1.15 mU/L

Incubation

2 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Porcine Insulin

100 %

Porcine C-peptide

< 0.001 %

Porcine Proinsulin

< 0.2 %

Human Insulin

108 %

Human C-peptide

< 0.01 %

Human Proinsulin

< 0.1 %

NovoRapid

3.2 %

Levemir

Not detected

Lantus

27 %

Humalog

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36588	DfU - 10-1205-01 v. 9.0
36580	DfU - 10-1205-01 v. 9.0
35700	DfU - 10-1205-01 v. 9.0
35179	DfU - 10-1205-01 v. 8.0
35521	DfU - 10-1205-01 v. 8.0
32442	DfU - 10-1205-01 v. 8.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36588	CoA - 10-1205-01
36580	CoA - 10-1205-01
35700	CoA - 10-1205-01
35521	CoA - 10-1205-01
35179	CoA - 10-1205-01
32442	CoA - 10-1205-01

SAFETY DATA SHEET

references

Nolen-Walston, R. D., Kulp, J. C., Stefanovski, D., & van Eps, A. W. (2025). Evaluation of an Automated Fluorescence Enzyme Immunoassay for Quantification of Equine Insulin and Comparison to Five Other Immunoassays. Journal of veterinary internal medicine, 39(2), e70038. Read more >

Lindåse, S. et al. (2016). A modified oral sugar test for evaluation of insulin and glucose dynamics in horses. Acta Veterinaria Scandinavica, 58(S1), 64. Read more >

Kheder, M. H., Sillence, M. N., Bryant, L. M., & de Laat, M. A. (2017). The equine glucose-dependent insulinotropic polypeptide receptor: A potential therapeutic target for insulin dysregulation. Journal of Animal Science, 95(6), 2509–2516. Read more >

Kheder, M. H., Bailey, S. R., Dudley, K. J., Sillence, M. N., & de Laat, M. A. (2018). Equine glucagon-like peptide-1 receptor physiology. PeerJ, 2018(1), e4316. Read more >

Marycz, K., Kornicka, K., Szlapka-Kosarzewska, J., & Weiss, C. (2018). Excessive endoplasmic reticulum stress correlates with impaired mitochondrial dynamics, mitophagy and apoptosis, in liver and adipose tissue, but not in muscles in EMS horses. International Journal of Molecular Sciences, 19(1), 165. Read more >