MPO ELISA

(10-1176-01)

Kit format: 1 x 96 wells

Measurement range: 4.0 - 200 μg/L

Broad measurement range

The MPO ELISA is calibrated against a highly purified, fully validated, commercially available MPO preparation

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Description

The Mercodia MPO ELISA is a high quality enzyme immunoassay for the quantification of human myeloperoxidase (MPO) in plasma or serum.

Myeloperoxidase (MPO) is an iron-containing glycoprotein found in neutrophils and monocytes. It forms a tetrameric complex with a molecular weight of 150 kDa, composed of two glycosylated alpha chains (59-64 kDa) and two unglycosylated beta chains (14 kDa). Upon microbial invasion, MPO is released from neutrophil granules, converting hydrogen peroxide and chloride ions into a potent oxidizing agent called hypochlorous acid. MPO serves as a marker for airway inflammation in asthma and environmental irritant exposure. It’s also implicated in atherogenesis and a potential contributor to atherosclerosis. Elevated MPO levels in serum are linked to cardiovascular disease (CAD), making it valuable as an inflammatory marker to identify CAD patients at risk of cardiac events without myocardial necrosis.

Test characteristics

Species

Human

Samples

Plasma or serum

Sample volume

25 µL

Assay range

4.0 - 200 μg/L

Detection limit

≤ 3 μg/L

Incubation

1 h + 1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

TPO

< 0.01 %

CRP

< 0.01 %

EPO

3.53 %

Lysosyme

0.03 %

Elastase

0.12 %

a 1-antitrypsin

< 0.01 %

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36297	DfU - 10-1176-01 v. 14.0
35775	DfU - 10-1176-01 v. 14.0
33735	DfU - 10-1176-01 v. 13.0
35224	DfU - 10-1176-01 v. 13.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36297	CoA - 10-1176-01
35775	CoA - 10-1176-01
33735	CoA - 10-1176-01
35224	CoA - 10-1176-01

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references

Karakas, M. et al. (2012) Myeloperoxidase is associated with incident coronary heart disease independently of traditional risk factors: Results from the MONICA/KORA Augsburg study. J. Intern. Med. 271, 43–50. Read more >

Januszewski, A. S. et al. (2016) Shorter telomeres in adults with Type 1 diabetes correlate with diabetes duration, but only weakly with vascular function and risk factors. Diabetes Res. Clin. Pract. 117, 4–11. Read more >

Russell, M. et al. (2017) Determining myeloperoxidase activity and protein concentration in a single assay: Utility in biomarker and therapeutic studies. J. Immunol. Methods 449, 76–79. Read more >