Rat/Mouse Proinsulin ELISA

Rat/Mouse Proinsulin ELISA

(10-1232-01)

Kit format: 1 x 96 wells

Measurement range: 3.5 - 200 pmol/L

Specific for rat and mouse proinsulin I and II

No cross reactivity to insulin or C-peptide

Fast and easy-to-use

TALK TO A PERSON
Description

Mercodia Rat/Mouse Proinsulin ELISA

The Rat/Mouse Proinsulin ELISA is a method for the quantitative determination of proinsulin in rat and mouse serum, plasma, cell culture medium and cellular extracts.

Test characteristics
Species

Rat and Mouse

Sample types

Serum, plasma, cell culture medium, tissue homogenate

Sample volume

25 µL

Assay range

3.5 - 200 pmol/L

Detection limit

≤3 pmol/L

Incubation

2 h+ 30 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Rat proinsulin I

100%

Rat proinsulin II

145 %

Rat insulin

Not detected

Rat C-peptide

Not detected

Mouse insulin

Not detected

Mouse C-peptide

Not detected

Mouse proinsulin I

234 %

Mouse proinsulin II

100 %

Human insulin

Not detected

Human C-peptide

Not detected

Human proinsulin

0.5 %

directions for use Certificate of Analysis
directions for use
LOT. NO. DOCUMENT
36598 DfU - 10-1232-01 v.11.0
36433 DfU - 10-1232-01 v.11.0
36409 DfU - 10-1232-01 v.11.0
35591 DfU - 10-1232-01 v.11.0
Certificate of Analysis
LOT. NO. DOCUMENT
36598 CoA - 10-1232-01
36433 CoA - 10-1232-01
36409 CoA - 10-1232-01
35591 CoA - 10-1232-01
SAFETY DATA SHEET
references

Mehta, Z. B. et al. (2016). Changes in the expression of the type 2 diabetes-associated gene VPS13C in the β-cell are associated with glucose intolerance in humans and mice. American Journal of Physiology - Endocrinology and Metabolism, 311(2), E488–E507. Read more >

Shyr, Z. A., Wang, Z., York, N. W., Nichols, C. G., & Remedi, M. S. (2019). The role of membrane excitability in pancreatic β-cell glucotoxicity. Scientific Reports, 9(1), 6952. Read more >

Gilmore, R. et al. (2019). PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity. ELIFE, 8, e44528 Read more >

Question about the product? Contact us

Robert Almstedt

Customer Success Manager & Area Sales Manager Nordics

(+46) 72 292 15 08

Contact Me

"*" indicates required fields

This field is for validation purposes and should be left unchanged.
Kit box single pack 1

Rat/Mouse Proinsulin ELISA

(10-1232-01)

ASK A QUESTION OR BOOK A MEETING?
WE WILL HELP YOU

Robert Almstedt

Customer Success Manager & Area Sales Manager Nordics

(+46) 72 292 15 08

Contact Me

"*" indicates required fields

This field is for validation purposes and should be left unchanged.