Rat/Mouse Proinsulin ELISA

(10-1232-01)

Kit format: 1 x 96 wells

Measurement range: 3.5 - 200 pmol/L

Specific for rat and mouse proinsulin I and II

No cross reactivity to insulin or C-peptide

Fast and easy-to-use

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Description

Mercodia Rat/Mouse Proinsulin ELISA

The Rat/Mouse Proinsulin ELISA is a method for the quantitative determination of proinsulin in rat and mouse serum, plasma, cell culture medium and cellular extracts.

Test characteristics

Species

Rat and Mouse

Sample types

Serum, plasma, cell culture medium, tissue homogenate

Sample volume

25 µL

Assay range

3.5 - 200 pmol/L

Detection limit

≤3 pmol/L

Incubation

2 h+ 30 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Rat proinsulin I

100%

Rat proinsulin II

145 %

Rat insulin

Not detected

Rat C-peptide

Not detected

Mouse insulin

Not detected

Mouse C-peptide

Not detected

Mouse proinsulin I

234 %

Mouse proinsulin II

100 %

Human insulin

Not detected

Human C-peptide

Not detected

Human proinsulin

0.5 %

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36433	DfU - 10-1232-01 v.11.0
36409	DfU - 10-1232-01 v.11.0
35591	DfU - 10-1232-01 v.11.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36433	CoA - 10-1232-01
36409	CoA - 10-1232-01
35591	CoA - 10-1232-01

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references

Mehta, Z. B. et al. (2016). Changes in the expression of the type 2 diabetes-associated gene VPS13C in the β-cell are associated with glucose intolerance in humans and mice. American Journal of Physiology - Endocrinology and Metabolism, 311(2), E488–E507. Read more >

Shyr, Z. A., Wang, Z., York, N. W., Nichols, C. G., & Remedi, M. S. (2019). The role of membrane excitability in pancreatic β-cell glucotoxicity. Scientific Reports, 9(1), 6952. Read more >

Gilmore, R. et al. (2019). PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity. ELIFE, 8, e44528 Read more >